The DnaE polymerase from Deinococcus radiodurans features RecA-dependent DNA polymerase activity
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منابع مشابه
The DnaE polymerase from Deinococcus radiodurans features RecA-dependent DNA polymerase activity
We report in the present study on the catalytic properties of the Deinococcus radiodurans DNA polymerase III α subunit (αDr). The αDr enzyme was overexpressed in Escherichia coli, both in soluble form and as inclusion bodies. When purified from soluble protein extracts, αDr was found to be tightly associated with E. coli RNA polymerase, from which αDr could not be dissociated. On the contrary, ...
متن کاملLesion bypass activity of DNA polymerase A from the extremely radioresistant organism Deinococcus radiodurans.
The bacterium Deinococcus radiodurans survives extremely high exposure to ionizing radiation and extended periods of desiccation. Radiation at the survival doses is known to cause numerous DNA damage, such as hundreds of double strand breaks and single strand breaks, as well as damage of the nucleobases. The mechanisms of D. radiodurans to survive the depicted threats are still only beginning t...
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Cells exposed to DNA damaging agents, produce different types of structural changes in the chromosome. Repair of these lesions requires synthesis of new DNA molecules, catalysed by specific DNA polymerases. A putative DNA polymerase has been characterized, for its role in DNA damage repair and radiation resistance in D. radiodurans, a bacterium best known for its extraordinary resistance to ...
متن کاملDNA helicase activity of the RecD protein from Deinococcus radiodurans.
The bacterium Deinococcus radiodurans is extremely resistant to high levels of DNA-damaging agents, including gamma rays and ultraviolet light that can lead to double-stranded DNA breaks. Surprisingly, the organism does not appear to have a RecBCD enzyme, an enzyme that is critical for double-strand break repair in many other bacteria. The D. radiodurans genome does encode a protein whose close...
متن کاملThe RecA proteins of Deinococcus radiodurans and Escherichia coli promote DNA strand exchange via inverse pathways.
The RecA protein of Escherichia coli, and all filament-forming homologues identified to date, promote DNA strand exchange by a common, ordered pathway. A filament is first formed on single-stranded DNA, followed by uptake of the duplex substrate. These proteins are thereby targeted to single-strand gaps and tails where recombinational DNA repair is required. The observed course of DNA strand ex...
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ژورنال
عنوان ژورنال: Bioscience Reports
سال: 2016
ISSN: 0144-8463,1573-4935
DOI: 10.1042/bsr20160364